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. 2016 Jan 14;6:19379. doi: 10.1038/srep19379

Figure 3. Co-injection of apelin-13 with preconditioned PBMNCs induces development of new mature vessels in ischemic hindlimbs.

Figure 3

At POD 28, skeletal muscles were collected from ischemic hindlimbs, and the numbers of mature microvessels were calculated in cross-sections. (a) Representative images of microvessels in muscle cross-sections. Endothelial cells and vascular smooth muscle cells were visualized using DyLite488-conjugated Lectin (green) and Cy3-conjugated anti-α-SMA (red) antibodies, respectively. Scale bar = 50 μm. (b) Quantitative analysis of total microvessels (Lectin+) in ischemic limbs was performed in muscle cross sections. (c) Quantitative analysis of α-SMA+ microvessel density in ischemic limbs. (d) Quantitative analysis of microvessel diameter in ischemic limbs. (e) Quantitative analysis of microvessel diameter in non-ischemic limbs. *p < 0.01 vs. PBS and apelin; †p < 0.01 vs. apelin; ‡p < 0.01 vs. apelin + N-PBMNC; §p < 0.01 vs. H-PBMNC.