Table 2. Characterization of Paracin 1.7.
Treatment | Diameter of zone of inhibition (mm) | Antibacterial activity (%) |
---|---|---|
Temperature | ||
40 °C, 20 min | 17.34 ± 0.05b | 98.30 |
50 °C, 20 min | 17.32 ± 0.03b | 98.19 |
60 °C, 20 min | 17.23 ± 0.02b | 97.68 |
70 °C, 20 min | 17.25 ± 0.06b | 97.79 |
100 °C, 20 min | 16.83 ± 0.06c | 95.41 |
121 °C, 20 min | 14.55 ± 0.07d | 82.48 |
Control (37 °C) | 17.64 ± 0.05a | 100 |
pH | ||
3 | 46.43 ± 0.06c | 174.48 |
4 | 43.70 ± 0.07c | 164.22 |
5 | 41.25 ± 0.04c | 155.02 |
6 | 31.59 ± 0.02c | 118.71 |
7 | 21.15 ± 0.05b | 79.48 |
8 | 15.75 ± 0.06b | 59.19 |
9 | 0d | |
Control (6.5) | 26.61 ± 0.06a | 100 |
Enzymes | ||
Protease K | 31.08 ± 0.11e | 90.27 |
Trypsin | 31.61 ± 0.16d | 91.81 |
α- Amylase | 31.97 ± 0.07c | 92.86 |
Papainase | 32.39 ± 0.09b | 94.07 |
Control | 34.43 ± 0.10a | 100 |
Organic solvents | ||
Methanol | 26.51 ± 0.04e | 97.11 |
Ethanol | 26.65 ± 0.04d | 97.62 |
Acetone | 26.70 ± 0.05d | 97.80 |
Benzene | 26.79 ± 0.02c | 98.13 |
n-Butanol | 27.09 ± 0.02b | 99.23 |
Control | 27.30 ± 0.03a | 100 |
Same letters represent significant differences (p < 0.05) relative to the control.
Different letters represent significant differences (p > 0.05) relative to the control.