Table 1. Primer sequences.
| Gene | GenBank no | Product size (bp) | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|---|---|
| β-actin | NM_001101.3 | 173 | AGCCTCGCCTTTGCCGA | CTGGTGCCTGGGGCG |
| COL-I | XM_005257059 | 26 | TTTGTGGACCTCCGGCTC | AAGCAGAGCACTCGCCCT |
| ALP | NM_001632.4 | 170 | AGTGCAGCTCATACTCCATGC | GCGGTTCCAGAAGTCCGGGTT |
| BSP | NM_004967.3 | 199 | CTGGCACAGGGTATACAGGGTTAG | GCCTCTGTGCTGTTGGTACTGGT |
| Runx2 | NM_011514964 | 135 | TCCACACCATTAGGGACCATC | TGCTAATGCTTCGTGTTTCCA |
| OCN | NM_001199662 | 223 | AGGGCAGCGAGGTAGTGAAG | CTCCTGAAAGCCGATGTGGT |
Abbreviations: COL-I, collagen type I; ALP, alkaline phosphatase; BSP, bone sialoprotein; Runx2, runt-related transcription factor 2; OCN, osteocalcin.