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. 2016 Jan 25;12(1):e1005763. doi: 10.1371/journal.pgen.1005763

Fig 3. timp over-expression impairs cyst encapsulation without affecting cell fate acquisition.

Fig 3

(A, B) Ovarioles from control (no heat-shock) and experimental (daily heat-shock regime for 2 weeks post-eclosion) animals carrying UAS-timp and hs-Gal4. Arrows compare a normal egg chamber containing 16 germ cells with an abnormal, fused egg chamber encasing 32 germ cells. (C, D) Control (c587-Gal4) and experimental (c587-Gal4; UAS-timp) ovarioles grown at 18°C and then switched to 25°C for 9 days. Arrowheads indicate Lamin C-positive stalk cells. (E, F) Control (c587-Gal4) and experimental (c587-Gal4; UAS-timp) ovarioles grown at 18°C and aged 4 weeks at 25°C. Large, empty arrowheads show Mmp-1 accumulation from region 2. The ring of strong Mmp1 staining that coincides with the region contracting to pinch off a new egg chamber in controls is absent in timp-overexpressing germaria (small, empty arrowheads). Occasionally, the TF of experimental germaria show a prominent accumulation of Mmp1 staining, indicating that timp overexpression may affect Mmp1 localization in this region. Images can be composites of several focal planes.