Fig 6. Microarray results were confirmed by probing expression of 4 genes using quantitative real-time PCR from ventricles from mice at 3 mo of age.

Hspa1a (A) and Nppb (B) are both involved in stress and pathological responses and were increased in the BACHD mice. Expression of Kcnip2 (C), a voltage-gated potassium channel interacting protein responsive to changes in calcium, and Acot1 (D) an enzyme involved in fatty acid metabolism were decreased in BACHD ventricles. Data are reported as ratios of the target gene expression to Tbp, and are shown as the mean ± SEM, * P<0.05 for genotypic differences (n = 4 per group).