Fig 6. Intracellular toxicity of CdiA-CT_PA2462 and CdiA-CT_PA0041 in E. coli cells and protection by their cognate CdiI immunity proteins.

Production of (A) CdiA-CT_PA2462 and (B) CdiA-CT_PA0041 were repressed with 0.5% glucose (grey bars) or induced with 1% arabinose (black bars). Error bars represent the standard error of the mean from three independent experiments. C. Purified CdiA-CT_PA0041 and CdiI-His₆ proteins were mixed in vitro with Ni²⁺-NTA resin. Fractions were analyzed by SDS-PAGE and Coomassie blue staining. Control experiments with purified proteins incubated alone are shown on the left panel while CdiA-CT/CdiI-His₆ mixtures are shown on the right panel. Marker lane (kDa) is shown on the left.