Figure 5. Comparison of structural stability and mobility in secondary structure elements of psychro- and mesophilic DERAs.

(a,b) Sum over energies of rigid contacts between residues of each (a) α-helix and (b) β-strand of DERA_EC, DERA_CP, and DERA_SH and the rest of the protein without the respective secondary structure element as computed by CNA. The values are divided by the number of amino acids in the secondary structure element and thus represent an average value with standard error. (c) Relative B-factor distribution from blue (mean – SD) to red (mean + SD). The natural substrate is represented in CPK mode (phosphate group in red/orange, other atoms in yellow). For DERA_EC, PDBID 1JCL¹² has been used.