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. 2016 Jan 18;6:19360. doi: 10.1038/srep19360

Figure 4. PC1/3 and TLR9 translocate to the same vesicular structures immediately upon exposure of cells to CpG-ODN.

Figure 4

Confocal images of NR8383 cells double-labelled with anti-PC1/3 (green, Alexa488) and anti-TLR9 (red, Alexa546). (a) Untreated cells. (b–f′) Cells incubated with 100 nM CpG-ODN 2006 for the indicated durations. All of the confocal images are shown at the same scale, bar = 10 μm. (g,h) Immunogold electron micrographs, showing the coexistence of PC1/3 (small gold particles, 6 nM) and TLR9 (large gold particles, 12 nM) in the same endosome (g) and in the same lysosome (h) after incubation with 100 nM CpG-ODN 2006 for 6 h. Bar = 250 nM for both electron micrographs.