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. 2016 Jan 19;6:19455. doi: 10.1038/srep19455

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(A) The upper panel is the predicted miR-378 binding site with CLU3′UTR. The lower panel is the miR-378 sequence containing the putative sCLU binding site and its mutant sequence. (B) Dual luciferase assay was performed with co-transfection of miR-378 mimics and the reporter containing sCLU 3′-UTR wild-type versus mutant. (C) RT-PCR results showed that A549 cells had higher expression of miR-378 compared with A549/cDDP cells. The lower panel is the representative picture of DNA electrophoresis after PCR. (D) The Western blot analysis showed the protein level of sCLU in A549 cells and A549/cDDP cells. The lower panel showed the representative blots. All results are from three separate experiments. (*P < 0.05, ***P < 0.001. Full-length blots are presented in Supplement.).