Figure 1. Overview of isolation procedure.

Human brain biopsy or autopsy tissue was mechanically and enzymatically dissociated to achieve a single cell suspension. This suspension was strained and plated in neural precursor cell media for up to 24 hours. Floating and weakly attached cells were removed and replated into a new flask and used to generate neural precursor cell cultures. Microglial culture media was added to the original flask for 5–7 days and microglia were harvested and utilised for experimental procedures.