Figure 5. Microglial HLA-DR, DP, DQ expression is induced by IFNγ but not IL-1β or LPS.

Microglia were treated with 10 ng/mL IL-1β, LPS, IFNγ or vehicle (0.1% BSA in PBS) for 24 hours and immunostained for HLA-DR, DP, DQ and PU.1 (a). The percentage of HLA, DR, DP, DQ positive cells was determined (b). N = 3 (two epilepsy and one GBM). Scale bar = 50 μm. ***p < 0.001.