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. 2016 Jan 19;6:19465. doi: 10.1038/srep19465

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Copyright © 2016, Macmillan Publishers Limited

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Mature mouse oocytes were sequentially transferred to holding medium (HM), vitrification solution 1 (VS1), and vitrification solution 2 (VS2). After loading with different carriers, oocytes were immersed in liquid nitrogen. Oocytes were thawed by sequential transfer to thawing solution 1 (TS1), thawing solution 2 (TS2), and HM. After incubation in fertilization medium (Toyoda-Yokoyama-Hosoki medium (TYH)), oocytes were subjected to insemination. (A) Open pulled straw (OPS) protocol. (B) Nylon loop protocol. (C) Mini-drop protocol. (D) Procedure without freezing.