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. 2016 Jan 19;6:19465. doi: 10.1038/srep19465

Figure 4. Effect of duration of equilibration in VS1, VS2 and thawing solution (TS) on OCCs.

Figure 4

Oocytes were equilibrated in VS1 for 1 min, 2 min, or 3 min followed by equilibration in VS2. (A) Oocyte survival. (B) Fertilization. (C) Blastocyst formation. Oocytes were equilibrated in VS1 and then equilibrated in VS2 for 10 secs, 20 secs, or 30 secs. (D) Oocyte survival. (E) Fertilization. (F) Blastocyst formation. After freezing, oocytes were incubated in TS for 3 min, 5 min, or 7 min. (G) Oocyte survival. (H) Fertilization. (I) Blastocyst formation. Different superscript letters (a–c) indicate p < 0.01.