Figure 1.

(A) hESCs were differentiated as monolayer and were sorted at day 3 on their MESP1-mCherry expression. Positive and negative fractions were further differentiated and RNA was collected at sequential timepoints day 5, day 7, day 10, and day 14. (B,C) The efficiency of the cardiac differentiations was monitored by flow cytometry at day 14 of differentiation. NKX2-5-eGFP levels were highly increased in the MESP1-positive derived fraction, treated with Wnt-pathway inhibitor Xav939. MESP1-negative fractions, lacking Xav939 treatment, were showing almost no NKX2-5-eGFP expression levels * P < 0.05, ** P < 0.001. Error bars indicate SEM: standard error of the mean. N = 3.