Figure 8. Enriched signalling pathways were identified through KEGG pathway analysis.

(A) Pathway analysis was performed on the enriched genes, selected as described before. Pathways that are visualized have a P < 0.05. Gene count indicates the number of genes that could be identified for each enriched pathway. % indicates the percentile of the gene count from the total number of genes in the specific pathway. Numbers indicate the pathway-specific p-value. A comprehensive list of pathways can be found in Table S2. (B) Heatmap visualization of the enriched transcripts belonging to the Wnt signalling pathway, which is enriched in early cardiac committed progenitors, and the TGF-β signalling pathway, enriched in cardiac progenitors and cardiomyocytes. Heatmaps show averaged values from n = 3. (C) Profile Plots of enriched Wnt-pathway-related and EMT-related gene expression levels throughout cardiac differentiation. Normalized values are visualized on a global expression scale and averaged on n = 3.