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. 2016 Jan 18;6:19470. doi: 10.1038/srep19470

Figure 1. Ndrg1 knockout mice shows decreased serum levels of M-CSF, macrophage-producing cytokines and low macrophage population in serum.

Figure 1

(a) Establishment of Ndrg1 KO (−/−) mice. PCR analysis of genomic DNA isolated from tails of each group of mice (left). Western blot analysis of cell lysates prepared from MEFs derived from each group of mice (right). Full-length blot are presented in Supplementary Figure 1. (b) Comparison of body size (top) and weight (bottom) between male and female WT and Ndrg1 KO mice at 5 weeks of age (n = 5 per genotype). (c) Multiplex suspension array analysis of serum levels of various growth factors, cytokines and chemokines. Relative expression levels are presented. The data were normalized by the WT expression levels of each factor. (d) Flow cytometric analysis of monocyte and neutrophil populations in whole blood. The percentages of F4/80+, CD11b+ cells (monocytes) and Gr-1+, CD11b+ cells (neutrophils) in the blood are shown (n = 3 per genotype). Each bar is an average ± SD, **P < 0.01 versus WT mice (two-tailed Student’s t-test).