Figure 3. Luciferase activities of the bovine ACSL1 promoter constructs in two cell lines.

A series of plasmids containing 5′ unidirectional deletions of the promoter region of the ACSL1 gene (pGL3–1933, 1634, 1328, 1034, 730, 436, 325, 140, pGL3–80, and pGL3) fused in frame to luciferase gene were transfected into 3T3L1 and C2C12 cell lines. After 48 h, the cells were harvested for luciferase assay. Results are expressed as the mean ± standard deviation in arbitrary units based on the firefly luciferase activity normalized against the Renilla luciferase activity for triplicate transfections. The error bars denote the standard deviation. The unpaired Student’s t-test was used to detect significant differences. *P < 0.05 and **P < 0.01. The data shown are representative of two independent experiments. Positions −325 and −140 bp in the promoter are also shown.