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. 2016 Jan 19;6:19543. doi: 10.1038/srep19543

Figure 4. Rspo1 overexpression modulates the expression of male and female biased genes.

Figure 4

(A) Specific expression of meiotic marker (Scp3) were recorded in germ cells of Rspo1-OV-XY fish at 15 dah, suggesting the induction of female biased gonadal development in these XY fish. Strong follicular expression of Cyp19a1 (B) and Foxl2 (C) oocyte specific expression of Fig1α (D) and simultaneous reduction of Gsdf (E) in ovarian somatic cells were observed in adult Rspo1-OV-XY fish, demonstrating the female-biased gonadal development in XY fish (scale bar-50 μm). (F) Gsdf and Sox9a2 expression in control XX, control XY, and Rspo1-OV-XY fish at different stages of development. Marked decrease in the expression of Gsdf and Sox9a2 in Rspo1-OV-XY fish was observed at 50 and 150 dah. (G) Cyp19a1a and Dmrt1 expression in control XX, control XY, and Rspo1-OV-XY fish at different stages of development. Marked increase in the expression of Cyp19a1a and decrease in Dmrt1 expression in Rspo1-OV-XY fish was noticed at 10-150 dah. Data are shown as mean ± S.E., and expressed as relative abundance corrected for Ef1α. Different letters (a, b, c, etc.) above the bars indicate that these groups differ significantly from each other at p < 0.01.