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. 2016 Jan 19;6:19543. doi: 10.1038/srep19543

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Copyright © 2016, Macmillan Publishers Limited

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Testosterone (A) and oestradiol-17β (B) levels in whole embryos and gonads in control XX, control XY, and Rspo1-OV-XY fish, at different stages of development. Markedly reduced testosterone and simultaneously increased estradiol-17β levels in Rspo1-OV-XY fish, demonstrate the female-biased steroid production in Rspo1-OV-XY fish. Data are shown as mean ± S.E. Different letters (a, b, c, etc.) above the bars indicate that these groups differ significantly from each other at p < 0.01.