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. 2016 Jan 19;6:19543. doi: 10.1038/srep19543

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Copyright © 2016, Macmillan Publishers Limited

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(A) Assessment of Rspo1 binding potential to different male and female candidate genes. Expression plasmids of Dmy, mutant Dmy, Dmrt1, and Foxl2 were co-transfected with medaka Rspo1 (4.5 kb) promoter constructs, and relative luciferase activity (RLU) was measured after 48 hours. The RLUs of each expression plasmid (s) were plotted on Y-axis to prepare the graph. (B) Real-time analysis of Dmy expression in Rspo1-OV-XY fish. Marked decrease in Dmy expression was observed from 10 dah. Data are shown as mean ± S.E. of three independent experiments and different letters (a, b, c, etc.) above the bars indicate that these groups differ significantly from each other at p < 0.01.