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Urine (125 μL) was mixed with 125 pmol CAR-HHEd5, 50 pmol CAR-HNEd11 diluted in 50 μL of ammonium formate 5 M, and 75 μL of water (final volume 250 μL, pH 7.0). The data are expressed as the mean ± standard deviation, n = 9 men (blue bars) and 6 women (red bars).
