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. 2016 Jan 19;6:19309. doi: 10.1038/srep19309

Figure 6. Guide RNA/pre-mRNA-hybrid formation in the free and editosome-bound RNA folding states.

Figure 6

Background: SHAPE-derived 2D-fold of the CYb pre-mRNA. The binding sites of eight base-complementary DNA-oligonucleotides acting as quasi gRNAs are shown as thick lines. Colored bars indicate the change in RNaseH-based cleavage of the different DNA/pre-mRNA-hybrid molecules in the free and editosome-bound RNA folding states. Grey: RNaseH-cleavage in the free RNA folding state (=1). Yellow-red: >1–5-fold stimulus in the editosome-bound folding state. Black: no RNaseH cleavage. Errors are standard deviations (SD). A representative example of the data acquisition, normalization and quantification procedures is shown in Supplementary Fig. 8.