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. 2016 Jan 19;6:19447. doi: 10.1038/srep19447

Figure 3. The WCC regulates ketoconazole (KTC) sensitivity and mediates the adaptive response of csp-1 to KTC.

Figure 3

(A) KTC sensitivity test of the ∆wc-1 and ∆wc-2 mutants. Mycelial plugs were inoculated onto plates, with or without KTC, and incubated at 28 °C in the dark. Images of colonies were captured after 24 h. (B) Relative inhibition rates. The calculation followed the method described in Fig. 1B. pwc-1 = 0.0029, n = 3; pwc-2 = 0.00002, n = 3. (C) ChIP-qPCR analysis of the WC-2 enrichment at the csp-1 promoter. The csp-1 promoter region associated with WC-2 was precipitated by an anti-WC-2 antibody and quantitatively measured by quantitative real-time polymerase chain reaction (qRT-PCR). The ratio of the qRT-PCR results between the chromatin immunoprecipitation sample and its input sample was used to indicate the level of WC-2 enrichment at the csp-1 promoter. Means of the results from three independent experiments are shown. (D) csp-1 transcripts after KTC treatment were detected by qRT-PCR. Values shown are the means of three replicates. Standard deviations are indicated with error bars.