Figure 5. Expression patterns of Monoterpenoid Indole Alkaloids (MIAs) biosynthetic unigenes during bulb development.
(a) Biosynthetic pathways of MIAs are based on PlantCYC (9.5), Gidding et al.52, De Luca et al.53 and Verma et al.54. Enzymes for which corresponding genes have been isolated from other plants are shown in green next to the arrows. Two or more known steps between two intermediates, whose cDNA sequences were not isolated, are omitted here but indicated by two continuous arrows. Black arrows, secologanin and strictosidine biosynthesis; blue arrows, ajmaline and raucaffricine biosynthesis after the branch-point intermediate aglycone; pink arrows, tabersonine-derived alkaloid biosynthesis after the branch-point intermediate aglycone, represented by vindoline and vinblastine biosynthesis. Full names of enzymes represented by their abbreviated names can be found in Table S1. (b) Expression patterns of representative DEGs. Illustrations are provided in Fig. 4b.