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. 2016 Jan 19;6:19338. doi: 10.1038/srep19338

Figure 3. SDS-PAGE analysis of conjugation at optimal condition.

Figure 3

(a) SDS-PAGE analysis of Ubi scFv-LPETG5-eGFP. M: BluEye Prestained Protein Ladder, 1: Conjugation of 5 μM Ubi scFv-LPETGG and 5 μM G5-eGFP aided by 5 μM SrtA, 2: Reaction control (5 μM Ubi scFv-LPETGG + 5 μM G5-eGFP), 3: Reaction control: (5 μM G5-eGFP + 5 μM SrtA), 4: Reaction control: (5 μM Ubi scFv-LPETGG + 5 μM SrtA) (b) SDS-PAGE analysis of Ubi scFv-LPETG5-invB. M: BluElf Prestained Protein Ladder, 1: Conjugation of 5 μM Ubi scFv-LPETGG and 5 μM G5-invB aided by 5 μM SrtA, 2: Reaction control (5 μM Ubi scFv-LPETGG + 5 μM G5-invB), 3: Reaction control: (5 μM Ubi scFv-LPETGG + 5 μM SrtA), 4: Reaction control: (5 μM G5-invB + 5 μM SrtA).