Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 19;6:19338. doi: 10.1038/srep19338

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Purification of Ubi scFv-LPETG₅-eGFP (i) His-tag purification of Ubi scFv-LPETG₅-eGFP. M: BluElf Prestained Protein Ladder, 1: Conjugation fraction of Ubi scFv-LPETGG and G₅-eGFP, 2: Flow through fraction of his-tag purification, 3: Wash fraction of his-tag purification, 4,5,6: Eluted purified fraction of Ubi scFv-LPETG₅-eGFP (ii) Avidin Purification of his-tag purified Ubi scFv-LPETG₅-eGFP. M: BluElf Prestained Protein Ladder,1: Conjugation fraction of Ubi scFv-LPETGG and G₅-eGFP, 2: Flow through fraction of Avidin purification, 3: Wash fraction of Avidin purification, 4: Eluted purified fraction of Ubi scFv-LPETG₅-eGFP. Figure 6(b) Purification of Ubi scFv-LPETG₅-invB. (i) His-tag purification of Ubi scFv-LPETG₅-invB. M: BluElf Prestained Protein Ladder, 1: Conjugation fraction of Ubi scFv-LPETGG and G₅-invB, 2: Flow through fraction of his-tag purification, 3: Wash fraction of his-tag purification, 4,5,6: Eluted purified fraction of Ubi scFv-LPETG₅-invB. (ii) Avidin Purification of his-tag purified Ubi scFv-LPETG₅-invB. M: BluElf Prestained Protein Ladder,1: Conjugation fraction of Ubi scFv-LPETGG and G₅-invB, 2: Flow through fraction of Avidin purification, 3: Wash fraction of Avidin purification, 4: Eluted purified fraction of Ubi scFv-LPETG₅-invB.