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. 2016 Jan 19;6:19338. doi: 10.1038/srep19338

Figure 6. Western blot analysis of purified Ubi scFv-LPETG5-eGFP and Ubi scFv-LPETG5-invB.

Figure 6

(a) Analysis of Ubi scFv-LPETG5-eGFP (i) against anti-His, M: BluElf Prestained protein ladder 1: control (eGFP), 2: conjugation reaction of Ubi scFv-LPETGG and G5-eGFP, 3: purified conjugated product, (ii) against protein-L M: BluElf Prestained Protein Ladder, 1: conjugation reaction of Ubi scFv-LPETGG and G5-eGFP, 2: double-purified conjugated product, (b) Analysis of Ubi scFv-LPETG5-invB (i) against anti-His M: BluElf Prestained Protein Ladder, 1: control: invB, 2: His-tag purification of Ubi scFv-LPETG5-invB, 3: double-purified conjugated product, (ii) against protein-L, 1: control: Ubi scFv, 2: His-tag purification of Ubi scFv-LPETG5-invB, 3: double-purified conjugated product.