. 2016 Jan 21;6:19211. doi: 10.1038/srep19211

Table 1. Mutation of the “headlock” motif leads to an increased dissociation. The table summarizes affinities (K_D), association (k_on) and dissociation constants (k_off) determined for BC2-Nb, BC2-Nb_R106E and BC2-Nb_R106S. Corresponding sensograms are shown in Supplementary Figure 3.

Nbs	R_max [RU]	K_D [nM]	k_on [M⁻¹s⁻¹]	k_off [s⁻¹]	Chi²
BC2-Nb	170 ± 0.34	1.4 ± 0.06	4.6 ± 0.04 × 10⁵	6.4 ± 0.27 × 10⁻⁴	3.5
BC2-Nb_R106E	160 ± 0.48	12.0 ± 0.15	2.8 ± 0.01 × 10⁵	3.4 ± 0.04 × 10⁻³	3.8
BC2-Nb_R106S	160 ± 0.43	9.7 ± 0.13	3.3 ± 0.03 × 10⁵	3.2 ± 0.03 × 10⁻³	3.1

Table 1. Mutation of the “headlock” motif leads to an increased dissociation. The table summarizes affinities (KD), association (kon) and dissociation constants (koff) determined for BC2-Nb, BC2-NbR106E and BC2-NbR106S. Corresponding sensograms are shown in Supplementary Figure 3.