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. 2016 Jan 22;6:19793. doi: 10.1038/srep19793

Figure 6. Reciprocal regulation of p53 and NFAT.

Figure 6

(A) HCT116 cells were left untreated (Control) or treated with AS, or transfected with a control shRNA construct, or AS plus a control shRNA construct, p53 shRNA, or AS plus p53 shRNA. After 24 hours of treatment cell lysates were prepared and probed with antibody against NFATc3, PML, p53 and β-actin. (B) HCT116 cells were left untreated (Control) or treated with AS, or transfected with p53 shRNA, or AS plus p53 shRNA. After 24 hours of treatment cell lysates were prepared and probed with antibody against p53, NFATc4 and β-actin. (C) HCT116 cells were left untreated, or transfected with a empty vector, or with pcDNA3.1-p53 overexpression construct. After 24 hours of treatment cell lysates were prepared and probed with antibody against p53, NFATc3 and β-actin. (D) Same treatment as in C and lysates probed with antibody against p53, NFATc4 and β-actin. (E) RKO and HCT116 cells were untreated or transfected with a NFATc3 shRNA construct and lysates probed with antibody against p53, MDM2, NFATc3 and β-actin. (F) HCT116 cells were untreated or transfected with an empty vector or NFATc3 shRNA construct and lysates probed with antibody against NFATc3, NFATc4, and β-actin. (G) HCT116 cells were left untreated, transfected with an empty vector or NFATc2 shRNA construct, lysates were probed with antibody against NFATc2, p53, NFATc4, and β-actin. (H) HCT116 cells were left untreated, transfected with an empty vector or p53 shRNA construct, lysates were probed with antibody against p53, NFATc1 and β-actin. kDa, molecular weight.