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. 2016 Jan 21;6:19715. doi: 10.1038/srep19715

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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TYLCV-infected 967 tomatoes were grown at normal (regime, 1: 22–25 ^oC/18–20 ^oC, day/night) and at high temperatures (regime 2: 40–45 ^oC/20–25 ^oC, day/night). Native proteins, extracted from those tomatoes at 21 and 28 dpi, were subjected to ultracentrifugation on sucrose gradients, which were subsequently divided in 10 fractions; aliquots were analyzed by western blots with antibodies against CP and HSP70 (used as a plant protein marker).