Figure 4. Change in localization of symbiosis establishment during development.

(a–c) After 24 h exposure to symbionts (100,000 algal cells/ml), larvae 48 hpf and 10 dpf were scored for infection efficiency and average number of algal cells per larva. n = 3 replicate experiments (a). Additionally, the localization of the algal cells in the aboral or oral endoderm in each larva was recorded: representative DIC microscopy images are shown in (b) and quantification in (c). n = 3 replicate experiments, ≥30 larvae per experiment. Error bars are SEM, *p < 0.05 as determined by Student’s t-test for unpaired data.