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. 2016 Jan 25;6:19821. doi: 10.1038/srep19821

Figure 5. Tyr-127 is critical for PP2Ac catalytic function.

Figure 5

(a) LC-ESI tandem MS (MS/MS) analysis by Q Exactive detected six peptides sequenced in the spectra. Summary of the PP2Ac modification sites (left). The amino acid sequence is based on NCBI accession number (NP_002706.1) for the mature PP2Acα protein. The numbering of all amino acid residues cited in this report refers to this amino acid sequence of the mature protein (right). (b) The figure is based on the secondary structure, deposited as PDB file (PDB accession code: 2IE4C). The helixes are colored orange, and the β-strands and loop switches are colored blue and grey, respectively. (c–g) Ribbon diagram of PP2Ac, with residues identified for PP2Ac nitration shown in yellow. (h) HUVECs were treated with TGF-β1, TGF-β1 + TAT-Y127WT/Y130WT/Y265WT Y267WT/Y284WT and cultured as untreated controls for 72 h. Representative western blot of VE-cadherin and α-SMA in each group with densitometry analysis (n ≥ 3).