Table 3. The gradient of mobile phase.
| Time(min) | Flow rate (mL/min) | A (%) | B (%) |
|---|---|---|---|
| 0 | 0.4 | 95 | 5 |
| 2 | 0.4 | 95 | 5 |
| 17 | 0.4 | 5 | 95 |
| 19 | 0.4 | 5 | 95 |
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