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. 2016 Jan 25;6:19480. doi: 10.1038/srep19480

Figure 2. Effect of Dmy knockdown on gonad development.

Figure 2

(A) The total number of mitotic germ cells counted (control XX, white; Dmy-KD XY, grey; control XY, black) at 5 dah (5H) and 10 dah (10H), was used as a marker for gonadal sex. Data are presented as mean of 10 individuals from both control and Dmy-KD groups. Error bars indicate SEM. Letters (a-c) above the bars indicate that these groups differ significantly (p < 0.05) from each other. (B–G) The candidate HE-stained sections of control XX females (B,E), control XY males (C,F), and Dmy-KD XY fish (D,G). Female-like gonad development is seen in Dmy-KD XY fish, which later forms a fully developed ovary. Note: B, C, and D represent gonads at 10 dah, while E, F, and G represent gonadal structure at 50 dah. (H,I) Dmy knockdown directed femininity was persistent during adulthood, which was confirmed by female-like external appearances (H) and gonad structure (I). The femininity was further confirmed by ISH with female (Cyp19a1, J; Foxl2, L; Rspo1, N), and male (Dmy, K; Cyp11a, M); Cyp11b, O) associated genes. (Scale bars, 20 μm.)