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. 2016 Jan 25;6:19480. doi: 10.1038/srep19480

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Copyright © 2016, Macmillan Publishers Limited

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(A,B) Ontogenic changes in the expression of male (Dmy, Gsdf, Sox9a2, and Dmrt1) (A) and female (Rspo1, Foxl2, and Spo11) (B) associated genes from 5 daf (5F) to 10 dah (10H) were analysed by real-time PCR. Data were normalised with Ef1a expression. Each column in the graph represents mean ± SEM of 10 individuals of control XY (white columns) and Dmy-knockdown XY (grey columns) fish. Letters above the bars indicate that these groups differ significantly (p < 0.05) from each other.