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. 2016 Jan 21;6:19618. doi: 10.1038/srep19618

Figure 2. Aortas from AhR-deficient mice show increased active eNOS phosphorylation and NO content.

Figure 2

Lysates from thoracic aortas of AhR-deficient mice (AhR ko) and their wildtype littermates (wt) were prepared as described in the methods section and used for immunoblots with anti-phospho serine 1177 eNOS, which recognizes the analogous phosphorylation on serine 1176 in the mouse protein (P-eNOS S1176), and anti-eNOS (eNOS) antibodies and to determine the NO content. (a) Immunoblots, bracketed samples represent littermates. (b) Semiquantitative analysis, relative eNOS activity is the ratio of P-eNOS S1176 normalized to eNOS (c) NO content, wt animals were set to 1 (all data are mean ± SEM, n = 3 per group, *p < 0.05 vs. wt, unpaired Student’s t-test).