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. 2016 Jan 21;6:19618. doi: 10.1038/srep19618

Figure 4. AhR agonist treatment impairs eNOS activation and reduces S-NO content in human endothelial cells.

Figure 4

EC were treated for 18 h with 250 nM BaP (BaP), 2.5 μM MNF (MNF), a combination of both (BaP/MNF) or left untreated (con) and lysates were used for immunoblots with anti-phospho serine 1177 eNOS (P-eNOS S1177), anti-phospho threonine 495 eNOS (P-eNOS T495) and anti-eNOS (eNOS) antibodies and determination of S-NO content. (a) Representative Immunoblots. (b) Semiquantitative analysis, relative eNOS activity is the ratio of P-eNOS S1177 to P-eNOS T495 normalized to eNOS. (c) S-NO content, untreated cells were set to 1 (all data are mean ± SEM, n = 3 per group, *p < 0.05 vs. con, #p < 0.05 vs. BaP, paired Student’s t-test).