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. 2016 Jan 21;6:19618. doi: 10.1038/srep19618

Figure 5. AhR overexpression impairs eNOS activation and reduces S-NO content in human endothelial cells.

Figure 5

EC were transfected with an expression vector for human AhR with a C-terminal myc-tag (AhR) or pcDNA3.1/myc-His(-)B as the corresponding empty vector (EV). Lysates were used for immunoblots with anti-phospho serine 1177 eNOS (P-eNOS S1177), anti-phospho threonine 495 eNOS (P-eNOS T495), anti-eNOS (eNOS) and anti-myc tag (myc (AhR) antibodies, the latter to demonstrate expression of exogenously delivered AhR, and for determination of S-NO content. (a) Representative Immunoblots. (b) Semiquantitative analysis, relative eNOS activity is the ratio of P-eNOS S1177 to P-eNOS T495 normalized to eNOS. (c) S-NO content, EV-transfected cells were set to 1 (all data are mean ± SEM, n = 3–4 per group, *p < 0.05 vs. EV, paired Student’s t-test).