Figure 7.
AhR-deficiency extends C. elegans healthy life span. (a) Life span analysis. Kaplan-Meier survival analysis showing the fraction of animals alive (mean ± SEM, wt = 18.69 ± 0.31, ahr-1(ju145) = 21.04 ± 0.36, five independent replicates, animals/censored: wt = 307/78, ahr-1(ju145) = 299/102, p = 1.8 × 10−6 log-rank test between curves). (b) Health span analysis. Movement was set as health parameter and the fraction of animals moving was determined (mean ± SEM, wt = 16.30 ± 0.37, ahr-1(ju145) = 18.74 ± 0.40, three independent replicates, animals/censored: wt = 168/39, ahr-1(ju145) = 180/32, p = 1.7 × 10−5 log-rank test between curves). (c) Fraction of animals moving of fraction alive (mean ± SEM, data from animals shown in b). (d) Health span analysis. Relative pharynx pumping rate was set as health parameter and the number of pharyngeal bulb contractions was measured at different animal age (mean ± SEM normalized to day 3, three independent replicates, 10 animals/replicate, **p < 0.01 vs. day 3 of same genotype, #p < 0.05 vs. wt same day). (e) Health span analysis. Heat stress resistance was set as another health parameter. Survival of animals of different age was assessed at 35 °C (mean ± SEM, three independent replicates, 20 animals/replicate, #p < 0.01 vs. wt same day).