Figure 1. Cytosolic [Ca²⁺]_i concentrations are unchanged with maternal age.

(a) Comparison of MII egg numbers collected from young and naturally-aged mice. Note the marked decrease in egg yields with advancing maternal age (P < 0.0001). Data collected from 20 independent experiments and presented as average number per mouse (n = 87 aged mice, n = 37 young mice). (b) Top panel: Young aged eggs were imaged side-by-side for all experiments. Bottom panel: Representative ratiometric image of young and aged eggs injected with Calcium Green^TM-1 dextran and Rhodamine B dextran. (c) Quantification of cytosolic [Ca²⁺]_i in young (n = 34) and aged (n = 22) eggs (Ca²⁺ -containing media) revealed no difference with maternal age (P = 0.47). Data collected from 4 independent experiments. ns indicates not statistically significant. a.u. represents arbitrary units. Error bars are s.e.m.