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. 2016 Jan 25;6:19816. doi: 10.1038/srep19816

Figure 1. Slowed actin elongation by Tpm3.1 is not reversed in the presence of anti-Tpm compound TR100.

Figure 1

(a) Representative polymerisation curves for 7 μM G-actin (10% pyrene labelled) alone or in the presence of saturating amounts (14 μM) of Tpm3.1 in 100 mM KCl, 2 mM Tris-HCl pH 8.0, 2 mM MgCl2, 0.2 mM EGTA, 0.2 mM ATP, 0.2 mM DTT. Tpm3.1 was pre-incubated with 50 μM TR100 or 1% (v/v) DMSO (vehicle control) prior to mixing with G-actin. Data fitted to a one phase association exponential model. (b) Initial rates of elongation. Rate data shown is mean ± S.D., averaged from n = 4–6 repeats. (c) Critical concentration of ATP-actin in the absence and presence of saturating concentrations of Tpm3.1 pre-incubated with 50 μM TR100 or 1% (v/v) DMSO (vehicle control).