Figure 4. Co-expression of TASK-1 ‘hits’ from the alanine scan with TASK-3.
(a) Sequence alignment of the tip of the TASK-1 and TASK-3 cap. TASK-1 ‘hits’ are highlighted in bold. *conserved residues; #conservative amino acids. (b) TASK-3 was injected in Xenopus oocytes alone (TASK-3 WT), in the presence of TASK-1 WT or in presence of TASK-1 mutants. Changes in current amplitudes compared to TASK-3 WT were analyzed at +40 mV. *p < 0.05; **p < 0.01; ***p < 0.001. Unpaired Student’s T-Test. Significance was probed against TASK-3 WT. Data are presented as Mean ± SEM. The number of experiments are included in the bar graphs.