Figure 5. hTRPM4-eGFP is not affected by membrane stretch.

(a) Representative single channel trace of hTRPM4-eGFP without stretch applied to the patch. Pipette and bath contained 140 mM NaCl, 5 mM KCl, 10 μM CaCl₂ and 5 mM HEPES pH 7.5. (b) Representative single channel trace of hTRPM4-eGFP with –10 mmHg stretch applied to the patch. Pipette and bath contained the same solution as in (a). (c) Relative open probability histogram of hTRPM4-eGFP under varying amounts of stretch (0, −5, −10 and −20 mmHg) (n = 3–4, p > 0.05).