. 2016 Jan 20;6:19518. doi: 10.1038/srep19518

Table 1. Dissociation constants (K_d) of Δ-som and single fluorinated Δ-som analogues obtained by ¹⁵N,¹H-HSQC and direct ¹⁹F NMR titrations.

		PROTEIN ¹⁵N/¹H Tracked/μM	LIGAND ¹⁹F Tracked (Single-F)/μM
AGSKNFFWKTFTSS	Δ-som	103 ± 47	N/A
AGSKNFFWKTFTSS	F1 Δ-som	61 ± 47	109 ± 13
AGSKNFFWKTFTSS	F2 Δ-som	51 ± 30	27 ± 3
AGSKNFFWKTFTSS	F3 Δ-som	84 ± 53	97 ± 21

Phe residue labelled for ¹⁹F NMR K_d determination is BOLD and underlined in the sequence. Standard errors from the Levenberg-Marquardt fitting routine of equation (1) were taken as the uncertainties.

Table 1. Dissociation constants (Kd) of Δ-som and single fluorinated Δ-som analogues obtained by 15N,1H-HSQC and direct 19F NMR titrations.

Table 1. Dissociation constants (K_d) of Δ-som and single fluorinated Δ-som analogues obtained by ¹⁵N,¹H-HSQC and direct ¹⁹F NMR titrations.