Table 3. Dissociation constants (K_d) of single, double and triple alanine substituted Δ-som analogues calculated by ¹⁵N,¹H- HSQC.

AGSKNFFWKTFTSS	15N Tracked/μM
Δsom	103 ± 47
Phe6 → Ala Δ-som	199 ± 100
Phe7 → Ala Δ-som	123 ± 96
Phe11 → Ala Δ-som	261 ± 101
Phe6,7 → Ala Δ-som	242 ± 177
Phe6,11 → Ala Δ-som	587 ± 174
Phe7,11 → Ala Δ-som	412 ± 219
Phe6,7,11 → Ala Δ-som	Non binding

Standard errors from the Levenberg-Marquardt fitting routine of equation (1) were taken as the uncertainties.