Table 1. Characteristics of protein clusters calculated by fitting auto-correlation values to equation 4.

	Radius (nm)	Increase in molecule density	Calculated #PSF (nm)	Density (per μm2)	Average molecules per cluster
Af647-VAMP2	162.1 ± 10.4	1.95 ± 0.07	22.5 ± 1.4	112.2 ± 12.2	18.06
co-labeled Af532-BoNT/A-Hc	120.1 ± 11.2	1.32 ± 0.08	19.6 ± 1.7	220.2 ± 34.1	13.20
Af647-CTB co-labeled	84.9 ± 16.6	2.59 ± 0.33	16.0 ± 1.2	77.3 ± 11.0	4.54
Af532-BoNT/A-Hc	163.3 ± 34.2	0.83 ± 0.09	16.2 ± 1.4	154.4 ± 18.9	10.79

^#PSF = point spread function, 99% confidence intervals are shown.