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. 2016 Jan 22;6:19817. doi: 10.1038/srep19817

Figure 5. HFD fed CD4KO mice do not exhibit lymphatic dysfunction.

Figure 5

(a) Weights for CD4KO mice fed either a NCD or HFD for 10 weeks (n = 5/group, p < 0.05). (b) Quantification of peak nodal uptake of 99mTc in the popliteal lymph node. Data is represented as a fold change relative to the lean control group (n = 4 animals per group, p = NS). (c) Quantification of the rate of uptake of 99mTc to the popliteal lymph node (p = NS). (d) Quantification of migration of CD11c+/MHC-II+/CD45.1+ transplanted dendritic cells to the popliteal lymph node (n = 4–5 animals/group, p = NS). (e) Top panels: Representative images demonstrating co-localization of CD45 (green) and LYVE-1 (red) in hindlimb tissues of NCD and HFD fed CD4KO mice. Bottom panels: Representative images demonstrating co-localization of CD3 (green) and LYVE-1 (red) in hindlimb tissues of NCD and HFD fed CD4KO mice. (f) Quantification of perilymphatic CD45+ cells (n = 4 animals/group; 3–4HPF/animal, p = NS). (g) Quantification of perilymphatic CD3+ cells (n = 4 animals/group; 3–4HPF/animal, p = NS).