Figure 2. C. pneumoniae-infected monocytes display unsteady rolling on E-selectin.

Uninfected (mock) or infected (Cpn) monocytes were perfused at 1 dyn/cm² on micro-channels coated with E-selectin (A–C) and micro-channels with activated confluent aortic endothelium (D–F). The cellular interactions were recorded at 20 fps for 5 minutes. Representative rolling velocity profile (A); distribution of instantaneous velocities of monocyte rolling with median (B); and pause time with mean ± SEM (C) on E-selectin. Representative rolling velocity profile (D); instantaneous velocity frequency distribution (E); and instantaneous acceleration (F) on activated endothelium. The results are mean ± SD of one representative experiment (unless mentioned otherwise) performed in triplicate, and the experiments were repeated five times. The statistical significance in the parameters between the groups was shown as p value from t test.