Figure 6. LDL increases uniform rolling of infected monocytes on endothelium.

(A) Monocytes were infected with mock PBS or Chlamydial EB (MOI 1) for 4 h and further incubated for 4 h with 0 or 100 μg/ml of LDL. The cells were then re-suspended at a concentration of 0.5 million/ml in media and perfused on activated aortic endothelium at 1 dyn/cm², and the interactions were visualized by video microscopy. Rolling flux of uninfected (Mock) and infected (Cpn) conditions with or without LDL. The *, # and § denote statistically significant change in the parameters of the groups in comparison to mock, Cpn and mock + LDL respectively (p < 0.05, ANOVA). (B-D) Instantaneous velocity (B); pause time (C); and acceleration (D) of infected (Cpn) and infected with LDL treatment (Cpn + LDL) conditions are shown. The statistical significance in the parameters between the groups was shown as p value from t test (B,C). The results are either mean ± SEM (A-C) or relative frequency (D) of one representative experiment performed in triplicate, and all the experiments were repeated three times.