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. 2016 Jan 20;6:16993. doi: 10.1038/srep16993

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Copyright © 2016, Macmillan Publishers Limited

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(A) ELISA assay was employed to detect the expression of TGF-β in MSCs DMEM culture medium with or without fetal bovine serum (FBS). (B,C) The MSCs culture medium (DMEM with 10% FBS) treated with 10% charcoal (CM1) was used to observe their function to induce androgen-independent manner in LNCaP cells compared with the culture medium untreated with 10% charcoal (CM2). CCK8 and PI/Annexin V-FITC assay was used to examined the cell viability and apoptosis of LNCaP cells in an androgen-deprivation (AD) condition (*P < 0.05).